Arylsulfatase Activity of Mycobacterium avium, M. intracellulare, and M. scrofulaceum

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Identification of cytoplasmic membrane protein antigens of Mycobacterium avium, M. intracellulare, and M. scrofulaceum.

The cytoplasmic membrane isolated from representative strains of the Mycobacterium avium, M. intracellulare, and M. scrofulaceum (MAIS) group contained approximately 20 proteins, as identified by SDS - polyacrylamide gel electrophoresis. One membrane protein predominated, comprising up to 50% of the total membrane protein. This major cytoplasmic membrane protein (MCMP) had a molecular weight of...

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Restriction endonuclease analysis of members of the Mycobacterium avium-M. intracellulare-M. scrofulaceum serocomplex.

Organisms belonging to the Mycobacterium avium-M. intracellulare-M. scrofulaceum (MAIS) serocomplex were subjected to restriction endonuclease analysis (REA) with the enzymes BstEII, PvuII, and BclI. Substantial genetic heterogeneity was observed between members of an authenticated collection of the 31 serotypes. Serotypes 2 and 3 were indistinguishable, however, as were serotypes 5 and 10. No ...

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Arylsulfatase activity for differentiating Mycobacterium avium and Mycobacterium intracellulare.

Arylsulfatase activities (96-h reaction) of various strains of Mycobacterium avium and M. intracellulare, as identified by a DNA probe test, were measured. The enzyme activities of M. avium strains were significantly lower [corrected] than those of M. intracellulare strains (P less than 0.005 to P less than 0.025). The enzyme activities did not vary with serovar; that is, the activities of sero...

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Selective medium for the isolation and enumeration of Mycobacterium avium-intracellulare and M. scrofulaceum.

A medium for the selective isolation and enumeration of Mycobacterium avium-intracellulare and M. scrofulaceum (MAIS) was developed, based upon the ability of these mycobacteria to utilize Tween 80 as sole carbon source and grow optimally at pH 5.5 on a simple mineral salts medium. Representative MAIS strains had higher efficiencies of plating on the Tween 80 medium compared with Middlebrook 7H...

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Modification of Schaefer's procedure for serotyping of organisms of the Mycobacterium avium-M. intracellulare-M. scrofulaceum complex.

Modifications to the tube-agglutination procedure which Schaefer developed for serotyping of organisms of the Mycobacterium avium-M. intracellulare-M. scrofulaceum complex are proposed.

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ژورنال

عنوان ژورنال: International Journal of Systematic Bacteriology

سال: 1990

ISSN: 0020-7713,1465-2102

DOI: 10.1099/00207713-40-1-66